growth media ............................................................ 20
microorganism .............................................19, 21, 23
oxygen transfer coefficient..................................21–22
spectrophotometer.................................................... 20
steady-state technique............................................... 19
sterilization process................................................... 21
unsteady-state technique ....................................18–19
Double-chamber perfusion bioreactor ............... 168, 169
E
E. coli BL21 (DE3).......................................................224
Eppendorf BioBLU® series ............................................ 86
Exosomes................................................................ 83, 193
Extracellular matrix (ECM)...........................85, 167, 241
Extracellular vesicles (EVs)
bioreactor preparation ............................................196
CELLine bioreactor (see CELLine AD 1000
bioreactor flask)
hiPSC-NPCs............................................................194
hiPSCs (see Human induced pluripotent stem cells
(hiPSCs))
hMSCs (see Human mesenchymal stromal/stem cells
(hMSCs))
hUC-MSCs..............................................................194
HUVECs .................................................................194
isolation from collected media ...............................201
isolation from human stem cells grown in bioreactor
cultures.........................................................196
PBS-VW bioreactors ...............................................194
wave bioreactor .......................................................194
F
Fabrication protocol, thermoplastic microfluidic devices
biological and medical applications.......................... 27
chip design and preparation
electrochemical wet etching ............................... 32
photolithography ..........................................29–31
using AutoCAD software ................................... 31
drilling and planarization.......................................... 34
of electrodes
blank COP substrate.............................. 32–33, 36
Cr/Au etching .................................................... 33
design creation .................................................... 32
hot embossing .....................................................34–36
Market and Market reports ...................................... 28
materials..................................................................... 29
microfluidic device with electrodes .......................... 36
microfluidic device without electrodes ..............35–36
modification and durability ...................................... 28
optical properties....................................................... 28
thermo-compression bonding.................................. 35
FiberCell bioreactor......................................................184
Fibroblasts .....................................................................246
Flow cytometry analysis.................................................. 90
Fluid flow.......................................................................242
G
Gas transfer................................................................13, 17
Geltrex coating..............................................................201
Glucose consumption ..................................................... 85
Glucose consumption rate...........................149, 153–154
Good manufacturing practice (GMP) ........................... 84
H
HepG2 tissue model ............................................ 245, 248
hiPSC complete culture medium (hiPSC-CCM)........195
hiPSC-derived neural progenitor cells
(hiPSC-NPCs) .................................... 194, 202
hMSC complete culture medium
(hMSC-CCM) .................................... 195–196
Horseradish peroxidase (HRP) ....................................191
Human induced pluripotent stem cells (hiPSCs)
in BioBLU®............................................................... 93
chemically defined, serum-free expansion .........87–89
Corning’s spinner flasks......................................94–97
culture medium, preparation of ............................... 70
differentiation in planar and bioreactor
cultures.........................................................195
differentiation of NPC organoid in spinner
flasks .................................................... 197–198
enzymatic dissociation of........................................106
in Eppendorf’s Instrumented BioBLU®
0.3c.........................................................97–100
equipment and consumables .................................... 68
extracellular matrices, preparation of.................69–70
media and consumables ............................................ 68
microcarrier cultures
media and small molecules for ........................... 69
preparation of ................................................70–71
reagents and consumables for ............................ 69
Ns1u criterion...........................................................107
planar culture, culture and expansion in....... 196–197
process analytics ........................................................ 93
sampling and quality control......................... 100–104
screening for high cardiac differentiation
potency.....................................................72–73
single-use spinner flasks ............................................ 92
small molecules, preparation of................................ 70
in spinner flasks ......................................................... 93
in stirred single-use bioreactors..........................84–87
T75-flask-based inoculum production...............93–94
in T75-flasks .............................................................. 92
Human mesenchymal stromal/stem cells
(hMSCs)......................................................... 83
expansion in PBS-VW bioreactors ................ 199–200
BIOREACTORS IN STEM CELL BIOLOGY: METHODS AND PROTOCOLS
Index 269